cysI [2018-01-03 10:34:03]

sulfite reductase (NADPH2)

locus: BSU33430

pI: 5.49

mw: 64.63 kDa

protein length: 605 aa

gene length: 1815 bp

function: sulfite reduction

product: sulfite reductase (NADPH2)

essential: no

synonyms: yvgR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]

Gene

Coordinates: 3,430,598 → 3,432,313

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source [Pubmed|12169591]

Expression and Regulation

Operons

• genes: [gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]-[gene|5A99F57DF0DDF5A129C3702E97621232A8173C22|cysJ]
  description: [Pubmed|12169591]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12169591], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL]: activation, [Pubmed|12169591], in [regulon|FB37E9B7F7727915ADB18E52FD07A8773C7B30FC|CysL regulon]

  regulation

  • induced by sulfite, sulfate, and thiosulfate ([protein|search|CysL]) [Pubmed|12169591]

Biological materials

Mutant

• MGNA-B602 (yvgR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1601 NBRP B. subtilis, Japan]
• 1A802 ( ''cysI''::''kan''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A802&Search=1A802 BGSC]
• 1A934 ( ''cysI''::''kan''), [Pubmed|12169591], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A934&Search=1A934 BGSC]
• BKE33430 (''[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• BKE33430 (Δ[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC, downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA
• BKK33430 (Δ[gene|A4C9E4D1163B703A917BFCF97A5F575B19EDDD8D|cysI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTGAAAAACTCCTTTC, downstream forward: _UP4_TGAGAAAGAAAGACGAGAGA

References

• 12169591,12107147,11445163